Products Catalog

Specification

PCR Mastercycler ep realplex2 and realplex2 S
‡ These two-color systems
meet the needs of most
laboratories
(Emission 520/550nm)
‡ Designed for use with
SYBR Green assays,
FAM-labeled probes or
duplex probe reactions.
‡ High reproducibility is
possible at 10 ¼L low total
reaction volumes with calibrated
Eppendorf pipettes.
For lower volumes please
see next page for automated
real-time PCR set-up
‡ Comes with two-year
warranty
Mastercycler ep realplex4 and realplex4 S
‡ This four-color system
(Emission 520/550/580/605nm)
provides full flexibility for
multiplexing.
‡ High reproducibility is
possible at 10 ¼L low total
reaction volumes with calibrated
Eppendorf pipettes.
For lower volumes please
see next page for automated
real-time PCR set-up
‡ Comes with two-year
warranty
Excitation source 96 LEDs (470 nm)
Emission filters 520 / 550 nm (realplex2)
520 / 550 / 580 / 605 nm (realplex4)
Detector 1-channel photomultiplier tube (realplex2)
2-channel photomultiplier tubes (realplex4)
Dynamic range 9 orders of magnitude from starting copy number
Sensitivity d 50 fmol fluorescein
Thermo module
Sample capacity 96 x 0.2 ml PCR tubes or one 96 PCR plate
(unskirted, semi-skirted, skirted—as per SBS standard)
Temperature control range of block 4–99 °C
Degree range of gradient, maximum 1–20 °C (thermomodule Mastercycler ep)
1–24 °C (thermomodule Mastercycler ep S)
Temperature control range of gradient 30–99 °C
Temperature of lid 105 °C
Block homogeneity 20–72 °C ± 0.3 °C
90 °C ± 0.4 °C
Control accuracy ± 0.2 °C
Heating speed* approx. 4 °C/s (thermomodule Mastercycler ep)
approx. 6 °C/s (thermomodule Mastercycler ep S)
Cooling speed* approx. 3 °C/s (thermomodule Mastercycler ep)
approx. 4.5 °C/s (thermomodule Mastercycler ep S)
Complete system
Dimensions (W x D x H) 26 cm x 41 cm x 39.6 cm
Total weight (detection module) 24 kg (7 kg)
Voltage requirements 200–240 V, 50 Hz–60 Hz
Power consumption 800 W
* Measured at block
Mastercycler® ep realplex
The Mastercycler ep realplex is currently not available in
every country. For further information please contact your
local Eppendorf organisation.
Ordering information
Description Order no.
Mastercycler® ep realplex:
Mastercycler® ep realplex2, with aluminum block and two emission filters 6300 000.507
Mastercycler® ep realplex2 S, with silver block and two emission filters 6300 000.604
Mastercycler® ep realplex4, with aluminum block and four emission filters 6302 000.504
Mastercycler® ep realplex4 S, with silver block and four emission filters 6302 000.601
Optical detection module to upgrade Mastercyler ep gradient/S
realplex2 6300 000.000
realplex4 6302 000.008
System accessories
twin.tec PCR Plates, see page 199 or visit www.eppendorf.com
Service
Preventive maintenance contracts, see page 191
Special cycler versions
Mastercycler® ep realplex2 i 6300 000.957
Mastercycler® ep realplex4 i 6302 000.954
Technical specifications subject to change. Ordering information for other voltage options
can be found in the appendix on page 304. Put on Product PartnerRecommend to your friends